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cell death imaging assays  (Sartorius AG)


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    Sartorius AG cell death imaging assays
    Cell Death Imaging Assays, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 15884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+death+imaging+assays/Live+Cell+Analysis+Instruments/pm37575265-69-1-48
    Average 99 stars, based on 15884 article reviews
    cell death imaging assays - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Divergent functional outcomes of NLRP3 blockade downstream of multi-inflammasome activation: therapeutic implications for ALS.
    Article Snippet: .. For cell death imaging assays, PI (0.5-1 mg/ml) was added after nigericin addition and membrane permeability was measured longitudinally through time lapse imaging, normalized for confluency to account for either cell proliferation or cell death at 37° C and 5% CO2 using a cell culture incubator housed IncuCyte (Sartorius). ..

    Membrane:

    Article Title: Divergent functional outcomes of NLRP3 blockade downstream of multi-inflammasome activation: therapeutic implications for ALS.
    Article Snippet: .. For cell death imaging assays, PI (0.5-1 mg/ml) was added after nigericin addition and membrane permeability was measured longitudinally through time lapse imaging, normalized for confluency to account for either cell proliferation or cell death at 37° C and 5% CO2 using a cell culture incubator housed IncuCyte (Sartorius). ..

    Permeability:

    Article Title: Divergent functional outcomes of NLRP3 blockade downstream of multi-inflammasome activation: therapeutic implications for ALS.
    Article Snippet: .. For cell death imaging assays, PI (0.5-1 mg/ml) was added after nigericin addition and membrane permeability was measured longitudinally through time lapse imaging, normalized for confluency to account for either cell proliferation or cell death at 37° C and 5% CO2 using a cell culture incubator housed IncuCyte (Sartorius). ..

    Cell Culture:

    Article Title: Divergent functional outcomes of NLRP3 blockade downstream of multi-inflammasome activation: therapeutic implications for ALS.
    Article Snippet: .. For cell death imaging assays, PI (0.5-1 mg/ml) was added after nigericin addition and membrane permeability was measured longitudinally through time lapse imaging, normalized for confluency to account for either cell proliferation or cell death at 37° C and 5% CO2 using a cell culture incubator housed IncuCyte (Sartorius). ..



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    Effects of IL-37 on apoptosis and proliferation in human amniotic epithelial cells. (a) <t>Cell</t> proliferation between the IL-37 siRNA group and the si_NC group was measured by 5-ethynyl-20-deoxyuridine (EdU) incorporation assay. The blue color indicates the nuclei, and the red color represents the EdU-positive nuclei. Scale bar: 200 μ m. (b) Statistical analysis of EdU staining in the result (a). (c) The <t>Live/Death</t> cell <t>kit</t> was used to analyze the number of dead cells between the IL-37 siRNA group and the si_NC group. The blue color indicates the nuclei, and the green color represents the death cells. Scale bar: 200 μ m. (d) Statistical analysis of death cell rate in the result (c). (e) Cell apoptosis between the IL-37 siRNA group and the si_NC group was assessed by the flow cytometer. Representative pictures of flow cytometry for apoptosis rate. (f) Statistical analysis of flow cytometry in the result (e). (g) The apoptosis-related protein biomarkers were measured by western blot in each group. (h) Statistical analysis of western blot in the result (g). (i) The STAT3 signaling was measured by western blot between the si_NC group and the IL-37 siRNA group. (j) Statistical analysis of western blot in the result (i). ∗ p < 0.05 vs. si_NC; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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    Image Search Results


    Effects of IL-37 on apoptosis and proliferation in human amniotic epithelial cells. (a) Cell proliferation between the IL-37 siRNA group and the si_NC group was measured by 5-ethynyl-20-deoxyuridine (EdU) incorporation assay. The blue color indicates the nuclei, and the red color represents the EdU-positive nuclei. Scale bar: 200 μ m. (b) Statistical analysis of EdU staining in the result (a). (c) The Live/Death cell kit was used to analyze the number of dead cells between the IL-37 siRNA group and the si_NC group. The blue color indicates the nuclei, and the green color represents the death cells. Scale bar: 200 μ m. (d) Statistical analysis of death cell rate in the result (c). (e) Cell apoptosis between the IL-37 siRNA group and the si_NC group was assessed by the flow cytometer. Representative pictures of flow cytometry for apoptosis rate. (f) Statistical analysis of flow cytometry in the result (e). (g) The apoptosis-related protein biomarkers were measured by western blot in each group. (h) Statistical analysis of western blot in the result (g). (i) The STAT3 signaling was measured by western blot between the si_NC group and the IL-37 siRNA group. (j) Statistical analysis of western blot in the result (i). ∗ p < 0.05 vs. si_NC; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Journal: Mediators of Inflammation

    Article Title: IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF- κ B and IL-6/STAT3 Signaling Pathway

    doi: 10.1155/2020/1069563

    Figure Lengend Snippet: Effects of IL-37 on apoptosis and proliferation in human amniotic epithelial cells. (a) Cell proliferation between the IL-37 siRNA group and the si_NC group was measured by 5-ethynyl-20-deoxyuridine (EdU) incorporation assay. The blue color indicates the nuclei, and the red color represents the EdU-positive nuclei. Scale bar: 200 μ m. (b) Statistical analysis of EdU staining in the result (a). (c) The Live/Death cell kit was used to analyze the number of dead cells between the IL-37 siRNA group and the si_NC group. The blue color indicates the nuclei, and the green color represents the death cells. Scale bar: 200 μ m. (d) Statistical analysis of death cell rate in the result (c). (e) Cell apoptosis between the IL-37 siRNA group and the si_NC group was assessed by the flow cytometer. Representative pictures of flow cytometry for apoptosis rate. (f) Statistical analysis of flow cytometry in the result (e). (g) The apoptosis-related protein biomarkers were measured by western blot in each group. (h) Statistical analysis of western blot in the result (g). (i) The STAT3 signaling was measured by western blot between the si_NC group and the IL-37 siRNA group. (j) Statistical analysis of western blot in the result (i). ∗ p < 0.05 vs. si_NC; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Article Snippet: Two fluorescent probes (Live/Death Cell Imaging Kit, Invitrogen, USA) (1 drop/500 μ l medium) were used in each well and then incubated for 15 minutes prior to photography under fluorescence microscopy.

    Techniques: Staining, Flow Cytometry, Western Blot